Abstract
Objective
To compare cardiopulmonary variables and blood gas analytes in guinea pigs (Cavia porcellus) during anesthesia with and without abdominal carbon dioxide (CO2) insufflation at intra-abdominal pressures (IAPs) 4 and 6 mmHg, with and without
endotracheal intubation.
Study design
Prospective experimental trial.
Animals
A total of six intact female Hartley guinea pigs.
Methods
A crossover study with sequence randomization for IAP and intubation status was used.
The animals were sedated with intramuscular midazolam (1.5 mg kg–1) and buprenorphine (0.2 mg kg–1) and anesthetized with isoflurane, and an abdominal catheter was inserted for CO2 insufflation. Animals with endotracheal intubation were mechanically ventilated and
animals maintained using a facemask breathed spontaneously. After 15 minutes of insufflation,
the following variables were obtained at each IAP: pulse rate, respiratory rate, rectal
temperature, oxygen saturation, end-tidal CO2 (intubated only), peak inspiratory pressure (intubated only), noninvasive blood pressure
and blood gas and electrolyte values, with a rest period of 5 minutes between consecutive
IAPs. After 4 weeks, the procedure was repeated with the guinea pigs assigned the
opposite intubation status.
Results
Intubated guinea pigs had significantly higher pH and lower partial pressure of CO2 in cranial vena cava blood (PvCO2) than nonintubated guinea pigs. An IAP of 6 mmHg resulted in a significantly higher
PvCO2 (65.9 ± 19.0 mmHg; 8.8 ± 2.5 kPa) than at 0 (53.2 ± 17.2 mmHg; 7.1 ± 2.3 kPa) and
4 mmHg (52.6 ± 10.8 mmHg; 7.01 ± 1.4 kPa), mean ± standard deviation, with intubated
and nonintubated animals combined.
Conclusions and clinical relevance
Although the oral anatomy of guinea pigs makes endotracheal intubation difficult,
capnoperitoneum during anesthesia induces marked hypercapnia in the absence of mechanical
ventilation. An IAP of 4 mmHg should be further evaluated for laparoscopic procedures
in guinea pigs because hypercapnia may be less severe than with 6 mmHg.
Keywords
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Article Info
Publication History
Published online: October 26, 2022
Accepted:
October 18,
2022
Received:
February 4,
2022
Publication stage
In Press Journal Pre-ProofIdentification
Copyright
© 2022 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.
