Evaluation of three tidal volumes (10, 12 and 15 mL kg−1) in dogs for controlled mechanical ventilation assessed by volumetric capnography: a randomized clinical trial



      To evaluate three routinely used tidal volumes (VT; 10, 12 and 15 mL kg−1) for controlled mechanical ventilation (CMV) in lung-healthy anaesthetized dogs by assessing alveolar ventilation (VTalv) and dead space (DS).

      Study design

      Prospective, randomized clinical trial.


      A total of 36 client-owned dogs.


      Dogs were randomly allocated to a VT of 10 (G10), 12 (G12) or 15 (G15) mL kg−1. After induction CMV was started. End-tidal carbon dioxide tension was maintained at 4.7–5.3 kPa by changing the respiratory frequency (fR; 6<fR<30 breaths minute−1). After 29 minutes, cardiovascular and respiratory variables were recorded for 3 minutes using a multiparameter monitor, volumetric capnography (VCap) and a blood gas analyser. The ratios of VTalv to body weight (VTalv kg−1) and airway DS to VT (VDaw/VT), Bohr's DS (VDBohr), Enghoff's DS (VDBE) and the volume of expired carbon dioxide per breath (VTCO2,br) were calculated. Mean airway pressure (MawP), fR and peak inspiratory pressure (PIP) were recorded. Data were analysed using one-way anova and Student–Newman–Keuls tests with a statistical significance set at p<0.05.


      No differences were observed for demographic data and cardiovascular variables between groups. A total of three dogs were excluded because of technical difficulties and one because of fR>30. VTalv kg−1 (p=0.001) increased and VDBohr (p=0.002) decreased with greater VT. VTCO2,br (p=0.017) increased and VDaw/VT (p=0.006), VDBE (p=0.008) and fR (p=0.002) decreased between G10 and G15. PIP (p=0.013) was significantly higher in G15 compared with that in G10 and G12. No changes were observed in MawP.

      Conclusions and clinical relevance

      A VT of 15 mL kg−1 is most appropriate for CMV in lung-healthy dogs (as evaluated by respiratory mechanics and VCap) and does not impair cardiovascular variables.


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        • Astrom E.
        • Niklason L.
        • Drefeldt B.
        • et al.
        Partitioning of dead space–a method and reference values in the awake human.
        Eur Respir J. 2000; 16: 659-664
        • Benchetrit G.
        Breathing pattern in humans: diversity and individuality.
        Respir Physiol. 2000; 122: 123-129
        • Blankman P.
        • Shono A.
        • Hermans B.J.
        • et al.
        Detection of optimal PEEP for equal distribution of tidal volume by volumetric capnography and electrical impedance tomography during decreasing levels of PEEP in post cardiac-surgery patients.
        Br J Anaesth. 2016; 116: 862-869
        • Bohr C.
        Über die Lungenatmung.
        Centralblatt für Physiologie. 1887; 1 (German): 236-268
        • Conrad S.A.
        • Zhang S.
        • Arnold T.C.
        • et al.
        Protective effects of low respiratory frequency in experimental ventilator-associated lung injury.
        Crit Care Med. 2005; 33: 835-840
        • De Monte V.
        • Grasso S.
        • De Marzo C.
        • et al.
        Abstracts presented at the Association of Veterinary Anaesthetists Meeting, 24–26th September, 2014, Vienna, Austria.
        Vet Anaesth Analg. 2015; 42: A1-A40
        • Dreyfuss D.
        • Saumon G.
        Ventilator-induced lung injury: lessons from experimental studies.
        Am J Respir Crit Care Med. 1998; 157: 294-323
        • Enghoff H.
        Volumen inefficax. Bemerkung zur Frage des schädlichen Raumes.
        Uppsala Lak Forhandl. 1938; 44 (German): 191-218
        • Faul F.
        • Erdfelder E.
        • Buchner A.
        • Lang A.G.
        Statistical power analyses using G*Power 3.1: tests for correlation and regression analyses.
        Behav Res Methods. 2009; 41: 1149-1160
        • Fletcher R.
        • Jonson B.
        • Cumming G.
        • Brew J.
        The concept of deadspace with special reference to the single breath test for carbon dioxide.
        Br J Anaesth. 1981; 53: 77-88
        • Fowler W.S.
        Lung function studies; the respiratory dead space.
        Am J Physiol. 1948; 154: 405-416
        • Garcia-Delgado M.
        • Navarrete-Sanchez I.
        • Chamorro-Marin V.
        • et al.
        Alveolar overdistension as a cause of lung injury: differences among three animal species.
        Sci World J. 2012; 2012: 985923
        • Gattinoni L.
        • Protti A.
        • Caironi P.
        • Carlesso E.
        Ventilator-induced lung injury: the anatomical and physiological framework.
        Crit Care Med. 2010; 38: S539-S548
        • Gros G.
        in: Engelhardt W. Breves G. Physiologie der Haustiere. 2nd edn. Enke, Germany2004: 232 (German)
        • Hopper K.
        • Powell L.L.
        Basics of mechanical ventilation for dogs and cats.
        Vet Clin North Am Small Anim Pract. 2013; 43: 955-969
        • McDonell W.N.
        • Kerr C.L.
        Physiology, pathophysiology, and anesthetic management of patients with respiratory disease.
        in: Grimm K. Lamont L.A. Tranquilli W.J. Veterinary Anesthesia and Analgesia. The Fifth Edition of Lumb and Jones. John Wiley & Sons, Inc, USA2015: 541
        • McDonell W.N.
        • Kerr C.L.
        Physiology, pathophysiology, and anesthetic management of patients with respiratory disease.
        in: Grimm K. Lamont L.A. Tranquilli W.J. Veterinary Anesthesia and Analgesia. The Fifth Edition of Lumb and Jones. John Wiley & Sons, Inc, USA2015: 518
        • McMillan M.W.
        • Whitaker K.E.
        • Hughes D.
        • et al.
        Effect of body position on the arterial partial pressures of oxygen and carbon dioxide in spontaneously breathing, conscious dogs in an intensive care unit.
        J Vet Emerg Crit Care (San Antonio). 2009; 19: 564-570
        • Mosing M.
        • Staub L.
        • Moens Y.
        Comparison of two different methods for physiologic dead space measurements in ventilated dogs in a clinical setting.
        Vet Anaesth Analg. 2010; 37: 393-400
        • Pelosi P.
        • Croci M.
        • Ravagnan I.
        • et al.
        The effects of body mass on lung volumes, respiratory mechanics, and gas exchange during general anesthesia.
        Anesth Analg. 1998; 87: 654-660
        • Pesenti A.
        • Marcolin R.
        • Prato P.
        • et al.
        Mean airway pressure vs. positive end-expiratory pressure during mechanical ventilation.
        Crit Care Med. 1985; 13: 34-37
        • Pinheiro de Oliveira R.
        • Hetzel M.P.
        • dos Anjos Silva M.
        • et al.
        Mechanical ventilation with high tidal volume induces inflammation in patients without lung disease.
        Crit Care. 2010; 14: R39
        • Reller M.D.
        • Donovan E.F.
        • Kotagal U.R.
        Influence of airway pressure waveform on cardiac output during positive pressure ventilation of healthy newborn dogs.
        Pediatr Res. 1985; 19: 337-341
        • Richard J.C.
        • Maggiore S.M.
        • Jonson B.
        • et al.
        Influence of tidal volume on alveolar recruitment. Respective role of PEEP and a recruitment maneuver.
        Am J Respir Crit Care Med. 2001; 163: 1609-1613
        • Slutsky A.S.
        Ventilator-induced lung injury: from barotrauma to biotrauma.
        Respir Care. 2005; 50: 646-659
        • Staffieri F.
        • De Monte V.
        • De Marzo C.
        • et al.
        Alveolar recruiting maneuver in dogs under general anesthesia: effects on alveolar ventilation, gas exchange, and respiratory mechanics.
        Vet Res Commun. 2010; 34: S131-S134
        • Suarez-Sipmann F.
        • Bohm S.H.
        • Tusman G.
        Volumetric capnography: the time has come.
        Curr Opin Crit Care. 2014; 20: 333-339
        • Tusman G.
        • Scandurra A.
        • Bohm S.H.
        • et al.
        Model fitting of volumetric capnograms improves calculations of airway dead space and slope of phase III.
        J Clin Monit Comput. 2009; 23: 197-206
        • Tusman G.
        • Gogniat E.
        • Bohm S.H.
        • et al.
        Reference values for volumetric capnography-derived non-invasive parameters in healthy individuals.
        J Clin Monit Comput. 2013; 27: 281-288
        • Tusman G.
        • Sipmann F.S.
        • Bohm S.H.
        Rationale of dead space measurement by volumetric capnography.
        Anesth Analg. 2012; 114: 866-874
        • Zick G.
        • Elke G.
        • Becher T.
        • et al.
        Effect of PEEP and tidal volume on ventilation distribution and end-expiratory lung volume: a prospective experimental animal and pilot clinical study.
        PLoS One. 2013; 8: e72675