Abstract
Objective
To evaluate the effect of hyaluronidase on uptake, duration and speed of elimination
of xylazine–tiletamine–zolazepam administered in the subcutaneous fat over the dorsal
lumbar region of swine.
Study design
Blinded, randomized, crossover study.
Animals
Six healthy Landrace/Large White pigs weighing 132±24 kg (mean±standard deviation).
Methods
Animals were administered xylazine (1 mg kg−1) and tiletamine–zolazepam (8 mg kg−1) (control treatment, CON), or xylazine–tiletamine–zolazepam at the same doses with
hyaluronidase (400 IU) (treatment HYA). The treatments were administered into the
dorsal lumbar adipose tissue, 2.5–3.0 cm laterally from the spinous process of the
second lumbar vertebra. The latency, anesthesia and recovery periods were measured.
Heart rate, noninvasive systolic, diastolic, and mean arterial pressures, respiratory
rate, hemoglobin oxygen saturation and rectal temperature were recorded every 10 minutes
for up to 50 minutes.
Results
One animal in CON and one animal in HYA were responsive to stimulation and did not
allow safe handling. No significant difference was found between treatments for latency
(CON 11.3±5.9 minutes, HYA 7.4±5.1 minutes) and anesthesia (CON 53±53 minutes, HYA 49±38 minutes) periods. Recovery period was shorter in HYA (9±6 minutes) than in CON (32±16 minutes) (p < 0.05). Physiological variables were not significantly changed over time and were
within accepted normal clinical limits for the species in both treatments.
Conclusion and clinical relevance
Hyaluronidase (400 IU) administered into adipose tissue in pigs did not reduce the
latency and duration of dissociative anesthesia, but was associated with faster recovery.
Keywords
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Article info
Publication history
Published online: January 13, 2017
Accepted:
September 13,
2016
Received:
April 8,
2016
Identification
Copyright
© 2017 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.
