Abstract
Objective
To compare an estimate of blood loss obtained using measurements from the Hemocue
photometer with a standard estimate in dogs undergoing spinal surgery.
Study design
Prospective clinical study.
Animals
Twenty-nine client-owned dogs.
Methods
During surgery, blood and all lavage fluids were collected in the suction container
and on to swabs. To prevent blood clot formation in the suction container, 10 mL citrate-phosphate
dextrose adenine (CPDA) was added. At the end of the procedure, the total volume in
the suction container was recorded. It was shaken to ensure uniformity and a 5 mL
sample tested with the HemoCue photometer. Blood loss in the suction container was
calculated as follows: Blood in suction (mL) = volume in bottle (mL) × [suction haemoglobin
(Hb) concentration (g dL−1)/pre-operative Hb concentration (g dL−1)]. This volume was added to the estimated volume of blood on the swabs (weight of
soaked swabs minus that of dry swabs) to provide the Hemocue estimate of total blood
loss. A standard haemorrhage estimate was performed using the volume of fluid in the
suction container at the end of surgery in excess of the total volume of lavage fluid
available, minus 10 mL CPDA. This volume was added to the estimated volume of blood
on the swabs to provide the standard estimate of total blood loss. Data were analyzed
with a paired t-test. Retrospective power calculations demonstrated an 80% power to detect a mean
difference of 25 mL between the two methods with a level of significance of 0.05.
Results
There was no significant difference in calculated blood loss between the two methods
(p = 0.8, mean difference: −2 mL, 95% CI: −20 to 16 mL).
Conclusions and clinical relevance
The HemoCue may be used to help estimate blood loss in dogs undergoing spinal surgery.
Keywords
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References
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Article info
Publication history
Accepted:
September 16,
2009
Received:
August 13,
2009
Identification
Copyright
© 2010 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Inc. All rights reserved.
