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Urinary neutrophil gelatinase-associated lipocalin concentration changes after acute haemorrhage and colloid-mediated reperfusion in anaesthetized dogs

      Abstract

      Objective

      To determine changes in urine neutrophil gelatinase-associated lipocalin concentration (uNGAL) in anaesthetized Greyhound dogs that developed acute tubular damage following haemorrhage and resuscitation with colloid-based fluids.

      Study design

      Prospective experimental study.

      Animals

      Seven healthy adult entire male Greyhound dogs.

      Methods

      During isoflurane anaesthesia, approximately 50 mL kg-1 of blood was removed to maintain mean arterial pressure (MAP) =40 mmHg for 1 hour followed by gelatin-based colloid administration to maintain MAP =60 mmHg for 3 hours. Data, including oxygen extraction ratio and uNGAL, were collected before (T0) and immediately following (T1) haemorrhage, and hourly during reperfusion (T2–T4). After T4, dogs were euthanized and renal tissue was collected for histology. Statistical analysis was performed using repeated-measures one-way anova. Data are presented as means (95% confidence interval).

      Results

      Histology identified renal tubular epithelial damage in all dogs. Urine NGAL concentration increased from 12.1 (0–30.6) ng mL-1 at T0 to 122.0 (64.1–180.0) ng mL-1 by T3. Compared with T0, uNGAL was significantly higher at T3 (p = 0.016) and was increased 24-fold.

      Conclusions and clinical relevance

      Despite wide individual variation in baseline uNGAL, increases in uNGAL were observed in all dogs, suggesting that this biomarker has the potential to detect renal tubular injury following haemorrhage-induced hypotension and colloid-mediated reperfusion.

      Keywords

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      References

        • Bangert K
        • Rosenkilde N
        • Jorgensen JP
        • et al.
        Immunochemical determination of monomer, homodimer and total neutrophil gelatinase-associated lipocalin (Abstract).
        J Am Soc Nephrol. 2012; 23: 830A
        • Basile DP
        • Anderson MD
        • Sutton TA
        Pathophysiology of acute kidney injury.
        Compr Physiol. 2012; 2: 1303-1353
        • Bayer O
        • Reinhart K
        • Sakr Y
        • et al.
        Renal effects of synthetic colloids and crystalloids in patients with severe sepsis: a prospective sequential comparison.
        Crit Care Med. 2011; 39: 1335-1342
        • Behrend EN
        • Grauer GF
        • Mani I
        • et al.
        Hospital-acquired acute renal failure in dogs: 29 cases (1983–1992).
        J Am Vet Med Assoc. 1996; 208: 537-541
        • Bennett M
        • Dent CL
        • Ma Q
        • et al.
        Urine NGAL predicts severity of acute kidney injury after cardiac surgery: a prospective study.
        Clin J Am Soc Nephrol. 2008; 3: 665-673
        • Cai L
        • Rubin J
        • Han W
        • et al.
        The origin of multiple molecular forms in urine of HNL/NGAL.
        Clin J Am Soc Nephrol. 2010; 5: 2229-2235
        • Cilley RE
        • Polley Jr, TZ
        • Zwischenberger JB
        • et al.
        Independent measurement of oxygen consumption and oxygen delivery.
        J Surg Res. 1989; 47: 242-247
        • Clerico A
        • Galli C
        • Fortunato A
        • et al.
        Neutrophil gelatinase-associated lipocalin (NGAL) as biomarker of acute kidney injury: a review of the laboratory characteristics and clinical evidences.
        Clin Chem Lab Med. 2012; 50: 1505-1517
        • Cowgill LD
        • Langston C
        Acute kidney insufficiency.
        in: Bartges J Polzin DJ Nephrology and Urology of Small Animals. Wiley-Blackwell, USA2011: 472-523
        • Daure E
        • Belanger MC
        • Beauchamp G
        • et al.
        Elevation of neutrophil gelatinase-associated lipocalin (NGAL) in non-azotemic dogs with urinary tract infection.
        Res Vet Sci. 2013; 95: 1181-1185
        • De Loor J
        • Daminet S
        • Smets P
        • et al.
        Urinary biomarkers for acute kidney injury in dogs.
        J Vet Intern Med. 2013; 27: 998-1010
        • Devarajan P
        Update on mechanisms of ischemic acute kidney injury.
        J Am Soc Nephrol. 2006; 17: 1503-1520
        • Devarajan P
        Neutrophil gelatinase-associated lipocalin: a promising biomarker for human acute kidney injury.
        Biomark Med. 2010; 4: 265-280
        • DiBartola SP
        Applied renal physiology.
        in: Fluid, Electrolyte, and Acid-Base Disorders in Small Animal Practice. 4th edn. Elsevier Saunders, USA2012: 26-43
        • Goldstein SL
        Urinary kidney injury biomarkers and urine creatinine normalization: a false premise or not?.
        Kidney Int. 2010; 78: 433-435
        • Grenier FC
        • Ali S
        • Syed H
        • et al.
        Evaluation of the ARCHITECT urine NGAL assay: assay performance, specimen handling requirements and biological variability.
        Clin Biochem. 2010; 43: 615-620
        • Gutierrez G
        • Warley AR
        • Dantzker DR
        Oxygen delivery and utilization in hypothermic dogs.
        J Appl Physiol. 1986; 60: 751-757
        • Haase M
        • Bellomo R
        • Devarajan P
        • et al.
        Accuracy of neutrophil gelatinase-associated lipocalin (NGAL) in diagnosis and prognosis in acute kidney injury: a systematic review and meta-analysis.
        Am J Kidney Dis. 2009; 54: 1012-1024
        • Lee YJ
        • Hu YY
        • Lin YS
        • et al.
        Urine neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute canine kidney injury.
        BMC Vet Res. 2012; 8: 248
        • Lippi G
        • Cervellin G
        Neutrophil gelatinase-associated lipocalin: A more specific assay is needed for diagnosing renal injury.
        Clin Chim Acta. 2012; 413: 1160-1161
        • Mason DJ
        • O’Grady M
        • Woods JP
        • et al.
        Assessment of lithium dilution cardiac output as a technique for measurement of cardiac output in dogs.
        Am J Vet Res. 2001; 62: 1255-1261
        • Mishra J
        • Ma Q
        • Prada A
        • et al.
        Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury.
        J Am Soc Nephrol. 2003; 14: 2534-2543
        • Mishra J
        • Dent C
        • Tarabishi R
        • et al.
        Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery.
        Lancet. 2005; 365: 1231-1238
        • Moore EM
        • Bellomo R
        • Nichol AD
        The meaning of acute kidney injury and its relevance to intensive care and anaesthesia.
        Anaesth Intensive Care. 2012; 40: 929-948
        • Mori K
        • Nakao K
        Neutrophil gelatinase-associated lipocalin as the real-time indicator of active kidney damage.
        Kidney Int. 2007; 71: 967-970
        • Mori K
        • Lee HT
        • Rapoport D
        • et al.
        Endocytic delivery of lipocalin-siderophore-iron complex rescues the kidney from ischemia-reperfusion injury.
        J Clin Invest. 2005; 115: 610-621
        • Mugford A
        • Li R
        • Humm K
        Acute kidney injury in dogs and cats 1. Pathogenesis and diagnosis.
        In Practice. 2013; 35: 253-264
        • Rosenberger C
        • Rosen S
        • Heyman SN
        Renal parenchymal oxygenation and hypoxia adaptation in acute kidney injury.
        Clin Exp Pharmacol Physiol. 2006; 33: 980-988
        • Rosenthal RC
        Chemotherapy.
        in: Ettinger SJ Feldmann EC Textbook of Veterinary Internal Medicine, Diseases of the Dog and Cat. 4th edn. WB Saunders Company, USA1995: 473-483
        • Samsel RW
        • Nelson DP
        • Sanders WM
        • et al.
        Effect of endotoxin on systemic and skeletal muscle O2 extraction.
        J Appl Physiol. 1988; 65: 1377-1382
        • Schabinski F
        • Oishi J
        • Tuche F
        • et al.
        Effects of a predominantly hydroxyethyl starch (HES)-based and a predominantly non HES-based fluid therapy on renal function in surgical ICU patients.
        Intensive Care Med. 2009; 35: 1539-1547
        • Segev G
        • Palm C
        • LeRoy B
        • et al.
        Evaluation of neutrophil gelatinase-associated lipocalin as a marker of kidney injury in dogs.
        J Vet Intern Med. 2013; 27: 1362-1367
        • Smertka M
        • Chudek J
        Using NGAL as an early diagnostic test of acute kidney injury.
        Ren Fail. 2012; 34: 130-133
        • Steffey EP
        • Howland D
        Isoflurane potency in the dog and cat.
        Am J Vet Res. 1977; 38: 1833-1836
        • Steinbach S
        • Weis J
        • Schweighauser A
        • et al.
        Plasma and urine neutrophil gelatinase-associated lipocalin (NGAL) in dogs with acute kidney injury or chronic kidney disease.
        J Vet Intern Med. 2014; 28: 264-269
        • Valverde A
        • Morey TE
        • Hernandez J
        • et al.
        Validation of several types of noxious stimuli for use in determining the minimum alveolar concentration for inhalation anesthetics in dogs and rabbits.
        Am J Vet Res. 2003; 64: 957-962
        • Wagener G
        • Jan M
        • Kim M
        • et al.
        Association between increases in urinary neutrophil gelatinase-associated lipocalin and acute renal dysfunction after adult cardiac surgery.
        Anesthesiology. 2006; 105: 485-491
        • Waikar SS
        • Sabbisetti VS
        • Bonventre JV
        Normalization of urinary biomarkers to creatinine during changes in glomerular filtration rate.
        Kidney Int. 2010; 78: 486-494
        • Zaldívar-López S
        • Marín LM
        • Iazbik MC
        • et al.
        Clinical pathology of Greyhounds and other sighthounds.
        Vet Clin Pathol. 2011; 40: 414-425